产品描述
CASP3(半胱氨酸蛋白酶3)是一种在凋亡过程中发挥关键作用的蛋白酶,它在分子水平上参与了凋亡的启动和执行过程。
华美生物所提供的Rat Caspase 3,Casp-3 ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测大鼠血清、血浆、组织匀浆样本中的CASP3,其灵敏度为0.078 ng/mL,检测范围为0.312 ng/mL-20 ng/mL。
别名
Casp3 ELISA Kit; Cpp32Caspase-3 ELISA Kit; CASP-3 ELISA Kit; EC 3.4.22.56 ELISA Kit; Apopain ELISA Kit; Cysteine protease CPP32 ELISA Kit; CPP-32 ELISA Kit; IRP ELISA Kit; LICE ELISA Kit; Protein Yama ELISA Kit; SREBP cleavage activity 1 ELISA Kit; SCA-1) [Cleaved into: Caspase-3 subunit p17; Caspase-3 subunit p12] ELISA Kit
缩写
CASP3
Uniprot No
P55213
灵敏度
0.078 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cell Biology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat Casp-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
84 |
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| Range % |
80-92 |
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| 1:2 |
Average % |
98 |
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| Range % |
91-105 |
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| 1:4 |
Average % |
99 |
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| Range % |
92-110 |
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| 1:8 |
Average % |
91 |
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| Range % |
86-98 |
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回收率
| The recovery of rat Casp-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
89-98 |
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| EDTA plasma (n=4) |
96 |
90-100 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 20 |
2.915 |
2.903 |
2.909 |
2.828 |
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| 10 |
2.321 |
2.283 |
2.302 |
2.221 |
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| 5 |
1.467 |
1.444 |
1.456 |
1.375 |
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| 2.5 |
0.727 |
0.706 |
0.717 |
0.636 |
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| 1.25 |
0.365 |
0.359 |
0.362 |
0.281 |
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| 0.625 |
0.216 |
0.206 |
0.211 |
0.130 |
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| 0.312 |
0.137 |
0.129 |
0.133 |
0.052 |
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| 0 |
0.084 |
0.078 |
0.081 |
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货期
3-5 working days