产品描述
This Rat 6-keto-PGF1α ELISA Kit was designed for the quantitative measurement of Rat 6-keto-PGF1α protein in serum, plasma, tissue homogenates, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL.
别名
6-keto-PGF1α,6-keto-PGF1 alpha
缩写
6-keto-PGF1α
灵敏度
7.81 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat 6-keto-PGF1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 85 | |
| Range % | 80-90 | |
| 1:2 | Average % | 88 | |
| Range % | 82-95 | |
| 1:4 | Average % | 90 | |
| Range % | 85-95 | |
| 1:8 | Average % | 92 | |
| Range % | 84-97 | |
回收率
| The recovery of rat 6-keto-PGF1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 92 | 87-100 | |
| EDTA plasma (n=4) | 98 | 93-103 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 2000 | 2.151 | 2.105 | 2.128 | 2.036 | | | 1000 | 1.744 | 1.659 | 1.702 | 1.610 | | | 500 | 1.328 | 1.294 | 1.311 | 1.219 | | | 250 | 0.972 | 0.896 | 0.934 | 0.842 | | | 125 | 0.627 | 0.602 | 0.615 | 0.523 | | | 62.5 | 0.313 | 0.289 | 0.301 | 0.209 | | | 31.25 | 0.196 | 0.191 | 0.194 | 0.102 | | | 0 | 0.095 | 0.088 | 0.092 | | |
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货期
3-5 working days