产品描述
This Human CK-MM ELISA Kit was designed for the quantitative measurement of Human CK-MM protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 mU/mL-200 mU/mL and the sensitivity is 0.78 mU/mL.
缩写
CK-MM
灵敏度
0.78 mU/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Metabolism
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human CK-MM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
87 |
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| Range % |
81-93 |
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| 1:2 |
Average % |
111 |
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| Range % |
99-114 |
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| 1:4 |
Average % |
90 |
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| Range % |
83-94 |
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| 1:8 |
Average % |
92 |
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| Range % |
84-96 |
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回收率
| The recovery of human CK-MM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
90 |
83-95 |
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| EDTA plasma (n=4) |
104 |
91-107 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| mU/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 200 |
2.123 |
2.088 |
2.106 |
1.969 |
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| 100 |
1.665 |
1.634 |
1.650 |
1.513 |
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| 50 |
1.128 |
1.178 |
1.153 |
1.016 |
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| 25 |
0.660 |
0.642 |
0.651 |
0.514 |
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| 12.5 |
0.382 |
0.397 |
0.390 |
0.253 |
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| 6.25 |
0.251 |
0.244 |
0.248 |
0.111 |
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| 3.12 |
0.165 |
0.173 |
0.169 |
0.032 |
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| 0 |
0.136 |
0.138 |
0.137 |
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货期
3-5 working days