产品描述
CCL5,也称为RANTES(Regulated on Activation, Normal T cell Expressed and Secreted),是C-C趋化因子家族中的一种趋化因子。它由多种细胞产生,如T细胞、巨噬细胞、内皮细胞和平滑肌细胞等,并且能够吸引多种CCR1、CCR3和CCR5受体表达的细胞,如单核细胞、T细胞和巨噬细胞等。CCL5在多种炎症和免疫调节过程中发挥着重要作用,如艾滋病、动脉粥样硬化、炎症性肠病等。
华美生物所提供的Dog C-C motif chemokine 5(CCL5) ELISA kit属于ELISA检测试剂盒,采用双抗夹心法定量检测狗血清、血浆、组织匀浆样本中的CCL5,其灵敏度为0.081 ng/mL,检测范围为0.156 ng/mL-10 ng/mL。
别名
CCL5 ELISA kit; SCYA5C-C motif chemokine 5 ELISA kit; Small-inducible cytokine A5 ELISA kit; T-cell-specific protein RANTES ELISA kit
缩写
CCL5
Uniprot No
Q8HYS0
灵敏度
0.081 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of dog CCL5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
105 |
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| Range % |
97-110 |
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| 1:2 |
Average % |
101 |
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| Range % |
95-106 |
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| 1:4 |
Average % |
96 |
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| Range % |
87-101 |
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| 1:8 |
Average % |
102 |
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| Range % |
97-107 |
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回收率
| The recovery of dog CCL5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
104 |
96-108 |
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| EDTA plasma (n=4) |
99 |
91-104 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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CV |
| 10 |
2.658 |
2.600 |
2.629 |
2.510 |
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1.56% |
| 5 |
1.985 |
2.016 |
2.001 |
1.882 |
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1.10% |
| 2.5 |
1.348 |
1.369 |
1.359 |
1.240 |
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1.09% |
| 1.25 |
0.919 |
0.927 |
0.923 |
0.804 |
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0.61% |
| 0.625 |
0.502 |
0.523 |
0.513 |
0.394 |
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2.89% |
| 0.313 |
0.294 |
0.314 |
0.304 |
0.185 |
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4.65% |
| 0.156 |
0.212 |
0.219 |
0.216 |
0.097 |
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2.29% |
| 0 |
0.117 |
0.121 |
0.119 |
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2.38% |
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货期
3-5 working days