产品描述

This Human C10orf59 ELISA Kit was designed for the quantitative measurement of Human C10orf59 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 μg/mL-40 μg/mL and the sensitivity is 0.156 μg/mL .

别名

6530404N21Rik ELISA Kit; AI452315 ELISA Kit; AW060440 ELISA Kit; C10orf59 ELISA Kit; Chromosome 10 open reading frame 59 ELISA Kit; FLJ11218 ELISA Kit; HGNC:25641 ELISA Kit; Hypothetical protein LOC55328 ELISA Kit; MAO C ELISA Kit; MAO-C ELISA Kit; mMAO C ELISA Kit; Monoamine oxidase C ELISA Kit; Monoamine oxidase-C ELISA Kit; Renalase ELISA Kit; Renalase FAD dependent amine oxidase ELISA Kit; RNLS ELISA Kit; RNLS_HUMAN ELISA Kit

缩写

C10orf59

Uniprot No

Q5VYX0

灵敏度

0.156 μg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cell Biology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human RNLS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		97
		Range %		92-104
1:2		Average %		94
		Range %		89-99
1:4		Average %		92
		Range %		88-98
1:8		Average %		87
		Range %		82-94

回收率

The recovery of human RNLS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		86		82-94
EDTA plasma (n=4)		97		93-105

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

µg/ml OD1 OD2 Average Corrected 40 2.515 2.414 2.465 2.321 20 1.733 1.632 1.683 1.539 10 0.971 0.992 0.982 0.838 5 0.568 0.557 0.563 0.419 2.5 0.402 0.414 0.408 0.264 1.25 0.285 0.274 0.280 0.136 0.625 0.227 0.221 0.224 0.080 0 0.144 0.143 0.144

货期

3-5 working days