产品描述

This Human ATP6V0A2 ELISA Kit was designed for the quantitative measurement of Human ATP6V0A2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL.

别名

a2 ELISA Kit; A2V ATPase ELISA Kit; ARCL ELISA Kit; ATP6a2 ELISA Kit; ATP6N1D ELISA Kit; ATP6V0A2 ELISA Kit; ATPase, H+ transporting, lysosomal V0 subunit a isoform 2 ELISA Kit; ATPase, H+ transporting, lysosomal V0 subunit a2 ELISA Kit; Infantile malignant osteopetrosis ELISA Kit; J6B7 ELISA Kit; Lysosomal H(+) transporting ATPase V0 subunit a2 ELISA Kit; Lysosomal H(+)-transporting ATPase V0 subunit a2 ELISA Kit; regeneration and tolerance factor ELISA Kit; Stv1 ELISA Kit; TJ6 ELISA Kit; TJ6M ELISA Kit; TJ6s ELISA Kit; V ATPase 116 kDa isoform a2 ELISA Kit; V type proton ATPase 116 kDa subunit a ELISA Kit; V type proton ATPase 116 kDa subunit a isoform 2 ELISA Kit; V-ATPase 116 kDa isoform a2 ELISA Kit; V-type proton ATPase 116 kDa subunit a isoform 2 ELISA Kit; Vacuolar proton translocating ATPase 116 kDa subunit a ELISA Kit; Vacuolar proton translocating ATPase 116 kDa subunit a isoform 2 ELISA Kit; Vph1 ELISA Kit; VPP2_HUMAN ELISA Kit; WSS ELISA Kit

缩写

ATP6V0A2

Uniprot No

Q9Y487

灵敏度

7.81 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human ATP6V0A2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		91
		Range %		86-95
1:2		Average %		102
		Range %		97-107
1:4		Average %		91
		Range %		85-97
1:8		Average %		97
		Range %		91-103

回收率

The recovery of human ATP6V0A2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		95		89-98
EDTA plasma (n=4)		97		90-100

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected   2000 2.281 2.298 2.290 2.098   1000 1.615 1.642 1.629 1.437   500 0.995 0.954 0.975 0.783   250 0.634 0.626 0.630 0.438   125 0.468 0.449 0.459 0.267   62.5 0.358 0.337 0.348 0.156   31.25 0.287 0.298 0.293 0.101   0 0.191 0.193 0.192

货期

3-5 working days