产品描述

This Human BMI1 ELISA Kit was designed for the quantitative measurement of Human BMI1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 25 pg/mL-1600 pg/mL and the sensitivity is 6.25 pg/mL.

别名

B lymphoma Mo MLV insertion region (mouse) ELISA Kit; B lymphoma Mo MLV insertion region 1 homolog ELISA Kit; Bmi 1 ELISA Kit; BMI1 ELISA Kit; BMI1 polycomb ring finger oncogene ELISA Kit; BMI1_HUMAN ELISA Kit; Flvi 2/bmi 1 ELISA Kit; FLVI2/BMI1 ELISA Kit; MGC12685 ELISA Kit; Murine leukemia viral (bmi 1) oncogene homolog ELISA Kit; Oncogene BMI 1 ELISA Kit; PCGF 4 ELISA Kit; PCGF4 ELISA Kit; Polycomb complex protein BMI 1 ELISA Kit; Polycomb complex protein BMI-1 ELISA Kit; Polycomb group protein Bmi1 ELISA Kit; Polycomb group ring finger 4 ELISA Kit; Polycomb group RING finger protein 4 ELISA Kit; RING finger protein 51 ELISA Kit; RNF 51 ELISA Kit; RNF51 ELISA Kit

缩写

BMI1

Uniprot No

P35226

灵敏度

6.25 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cancer

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human BMI1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		89
		Range %		84-93
1:2		Average %		95
		Range %		90-100
1:4		Average %		103
		Range %		99-107
1:8		Average %		90
		Range %		85-95

回收率

The recovery of human BMI1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		85		80-89
EDTA plasma (n=4)		94		90-98

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected 1600 1.768 1.896 1.832 1.714 800 1.169 1.203 1.186 1.068 400 0.739 0.704 0.722 0.604 200 0.472 0.457 0.465 0.347 100 0.320 0.340 0.330 0.212 50 0.267 0.271 0.269 0.151 25 0.195 0.182 0.189 0.071 0 0.121 0.115 0.118

货期

3-5 working days