产品描述

This Human AZGP1 ELISA Kit was designed for the quantitative measurement of Human AZGP1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 ng/mL-500 ng/mL and the sensitivity is 1.95 ng/mL .

别名

Alpha 2 glycoprotein 1 zinc ELISA Kit; Alpha 2 glycoprotein 1 zinc binding ELISA Kit; Alpha 2 glycoprotein zinc ELISA Kit; Azgp1 ELISA Kit; ZA2G ELISA Kit; ZA2G_HUMAN ELISA Kit; ZAG ELISA Kit; Zinc alpha 2 glycoprotein precursor ELISA Kit; Zinc-alpha-2-glycoprotein ELISA Kit; Zn alpha 2 glycoprotein ELISA Kit; Zn alpha 2 GP ELISA Kit; Zn alpha2 glycoprotein ELISA Kit; Zn-alpha-2-glycoprotein ELISA Kit; Zn-alpha-2-GP ELISA Kit; ZNGP1 ELISA Kit

缩写

AZGP1

Uniprot No

P25311

灵敏度

1.95 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Immunology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human AZGP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:100		Average %		97
		Range %		92-105
1:200		Average %		97
		Range %		92-103
1:400		Average %		92
		Range %		83-98
1:800		Average %		90
		Range %		84-99

回收率

The recovery of human AZGP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		98		92-103
EDTA plasma (n=4)		93		85-98

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 500 2.524 2.325 2.425 2.239 250 2.023 1.951 1.987 1.801 125 1.575 1.531 1.553 1.367 62.5 1.134 1.086 1.110 0.924 31.2 0.716 0.659 0.688 0.502 15.6 0.408 0.404 0.406 0.220 7.8 0.259 0.241 0.250 0.064 0 0.192 0.179 0.186

货期

3-5 working days