产品描述
This Mouse BMP2 ELISA Kit was designed for the quantitative measurement of Mouse BMP2 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 pg/mL-100 pg/mL and the sensitivity is 0.39 pg/mL.
别名
Bmp2 ELISA Kit; Bmp-2 ELISA Kit; Bone morphogenetic protein 2 ELISA Kit; BMP-2 ELISA Kit; Bone morphogenetic protein 2A ELISA Kit; BMP-2A ELISA Kit
缩写
BMP2
Uniprot No
P21274
灵敏度
0.39 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse BMP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
97 |
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| Range % |
88-101 |
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| 1:2 |
Average % |
101 |
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| Range % |
95-105 |
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| 1:4 |
Average % |
90 |
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| Range % |
85-94 |
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| 1:8 |
Average % |
99 |
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| Range % |
91-103 |
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回收率
| The recovery of mouse BMP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
88 |
80-92 |
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| EDTA plasma (n=4) |
101 |
92-107 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 100 |
2.643 |
2.453 |
2.548 |
2.423 |
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| 50 |
2.024 |
1.976 |
2.000 |
1.875 |
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| 25 |
1.349 |
1.312 |
1.331 |
1.206 |
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| 12.5 |
0.742 |
0.724 |
0.733 |
0.608 |
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| 6.25 |
0.449 |
0.467 |
0.458 |
0.333 |
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| 3.12 |
0.307 |
0.300 |
0.304 |
0.179 |
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| 1.56 |
0.242 |
0.237 |
0.240 |
0.115 |
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| 0 |
0.126 |
0.124 |
0.125 |
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货期
3-5 working days