产品描述
CAT (Catalase)是一种抗氧化酶,负责将过氧化氢分解为氧气和水。CAT广泛分布于生物体内,尤其是在肝脏、肺部和红血球中含量较高。
华美生物所提供的Fish Catalase (CAT) ELISA kit属于ELISA检测试剂盒,采用竞争法定量检测鱼类血清、血浆样本中的CAT,其灵敏度为62.5 mU/mL,检测范围为125 mU/mL-2000 mU/mL。
缩写
CAT
Uniprot No
H2L8K1
灵敏度
62.5 mU/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of fish CAT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:100 |
Average % |
95 |
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| Range % |
91-99 |
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| 1:200 |
Average % |
103 |
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| Range % |
99-107 |
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| 1:400 |
Average % |
98 |
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| Range % |
93-105 |
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| 1:800 |
Average % |
92 |
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| Range % |
87-95 |
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回收率
| The recovery of fish CAT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
99 |
94-104 |
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| EDTA plasma (n=4) |
88 |
83-96 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| mU/ml |
OD1 |
OD2 |
Average |
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| 2000 |
0.091 |
0.089 |
0.090 |
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| 1000 |
0.242 |
0.257 |
0.250 |
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| 500 |
0.553 |
0.587 |
0.570 |
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| 250 |
0.913 |
0.980 |
0.947 |
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| 125 |
1.647 |
1.563 |
1.605 |
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| 0 |
2.381 |
2.319 |
2.350 |
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货期
3-5 working days