产品描述
This Human AQP3 ELISA Kit was designed for the quantitative measurement of Human AQP3 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL .
别名
AQP3; Aquaporin-3; AQP-3; Aquaglyceroporin-3
缩写
AQP3
Uniprot No
Q92482
灵敏度
0.078 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human AQP-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:5 | Average % | 93 | |
| Range % | 90-98 | |
| 1:10 | Average % | 90 | |
| Range % | 85-100 | |
| 1:20 | Average % | 95 | |
| Range % | 89-99 | |
| 1:40 | Average % | 90 | |
| Range % | 82-98 | |
回收率
| The recovery of human AQP-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 97 | 89-105 | |
| EDTA plasma (n=4) | 95 | 90-102 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 20 | 2.123 | 2.131 | 2.127 | 1.994 | | | 10 | 1.774 | 1.792 | 1.783 | 1.650 | | | 5 | 1.156 | 1.163 | 1.160 | 1.027 | | | 2.5 | 0.542 | 0.549 | 0.546 | 0.413 | | | 1.25 | 0.351 | 0.348 | 0.350 | 0.217 | | | 0.625 | 0.230 | 0.241 | 0.236 | 0.103 | | | 0.312 | 0.179 | 0.183 | 0.181 | 0.048 | | | 0 | 0.132 | 0.134 | 0.133 | | |
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货期
3-5 working days