产品描述

This Human ACE ELISA Kit was designed for the quantitative measurement of Human ACE protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 18.75 pg/mL-1200 pg/mL and the sensitivity is 4.7 pg/mL.

别名

ACE 1 ELISA Kit; ACE ELISA Kit; ACE T ELISA Kit; ACE_HUMAN ELISA Kit; ACE1 ELISA Kit; Angiotensin converting enzyme somatic isoform ELISA Kit; Angiotensin converting enzyme testis specific isoform ELISA Kit; Angiotensin I converting enzyme 1 ELISA Kit; Angiotensin I converting enzyme ELISA Kit; Angiotensin I converting enzyme peptidyl dipeptidase A 1 ELISA Kit; angiotensin I converting enzyme peptidyl-dipeptidase A 1 transcript ELISA Kit; Angiotensin-converting enzyme ELISA Kit; Carboxycathepsin ELISA Kit; CD 143 ELISA Kit; CD143 ELISA Kit; CD143 antigen ELISA Kit; DCP 1 ELISA Kit; DCP ELISA Kit; DCP1 ELISA Kit; Dipeptidyl carboxypeptidase 1 ELISA Kit; Dipeptidyl carboxypeptidase I ELISA Kit; Kininase II ELISA Kit; MGC26566 ELISA Kit; MVCD3 ELISA Kit; Peptidase P ELISA Kit; Peptidyl dipeptidase A ELISA Kit; soluble form ELISA Kit; Testicular ECA ELISA Kit

缩写

ACE

Uniprot No

P12821

灵敏度

4.7 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Neuroscience

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human ACEⅠ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:200		Average %		97
		Range %		92-104
1:400		Average %		91
		Range %		82-98
1:800		Average %		103
		Range %		99-111
1:1600		Average %		87
		Range %		82-96

回收率

The recovery of human ACEⅠ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		90		85-98
EDTA plasma (n=4)		112		105-117

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 1200 2.844 2.752 2.798 2.663 600 2.536 2.382 2.459 2.324 300 1.916 1.985 1.951 1.816 150 1.390 1.355 1.373 1.238 75 0.715 0.764 0.740 0.605 37.5 0.386 0.375 0.381 0.246 18.75 0.222 0.239 0.231 0.096 0 0.132 0.137 0.135

货期

3-5 working days