产品描述
This Rat Ang1-7 ELISA Kit was designed for the quantitative measurement of Rat Ang1-7 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 18.75 pg/mL-1200 pg/mL and the sensitivity is 4.68 pg/mL.
缩写
Ang1-7
灵敏度
4.68 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat Ang1-7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 94 | |
| Range % | 80-105 | |
| 1:2 | Average % | 96 | |
| Range % | 85-104 | |
| 1:4 | Average % | 102 | |
| Range % | 92-114 | |
| 1:8 | Average % | 99 | |
| Range % | 80-110 | |
回收率
| The recovery of rat Ang1-7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 96 | 83-112 | |
| EDTA plasma (n=4) | 95 | 90-102 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml | OD1 | OD2 | Average | Corrected | | | 1200 | 3.121 | 3.129 | 3.125 | 2.986 | | | 600 | 2.532 | 2.522 | 2.527 | 2.388 | | | 300 | 1.739 | 1.765 | 1.752 | 1.613 | | | 150 | 1.050 | 1.041 | 1.046 | 0.907 | | | 75 | 0.623 | 0.642 | 0.633 | 0.494 | | | 37.5 | 0.435 | 0.447 | 0.441 | 0.302 | | | 18.75 | 0.265 | 0.254 | 0.260 | 0.121 | | | 0 | 0.135 | 0.143 | 0.139 | | |
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货期
3-5 working days