产品描述
The Rat Bone morphogenetic protein 2 (BMP-2) ELISA Kit is a reliable and precise tool for the quantification of BMP-2 in rat serum, plasma, and tissue homogenates. BMP-2 is a protein that plays a critical role in signal transduction pathways，cardiogenesis, neurogenesis, and osteogenesis, particularly in bone formation and development. Our ELISA kit detects BMP-2 with high sensitivity, with a detection range of 7.8 pg/mL to 500 pg/mL and a sensitivity of 1.95 pg/mL.
The assay principle is a sandwich measurement, providing quantitative results that can be obtained in as little as 1-5 hours. The kit is optimized for use with a sample volume of 50-100ul, making it a cost-effective and efficient solution for researchers.
This ELISA kit is ideal for researchers investigating signal transduction pathways and the role of BMP-2 in bone development. Additionally, our kit has been cited in over four publications, demonstrating its reliability and accuracy.
别名
Bmp2 ELISA kit; Bmp-2Bone morphogenetic protein 2 ELISA kit; BMP-2 ELISA kit; Bone morphogenetic protein 2A ELISA kit; BMP-2A ELISA kit
缩写
BMP2
Uniprot No
P49001
灵敏度
1.95 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat BMP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 95 | |
| Range % | 95-110 | |
| 1:2 | Average % | 84 | |
| Range % | 80-88 | |
| 1:4 | Average % | 100 | |
| Range % | 95-106 | |
| 1:8 | Average % | 98 | |
| Range % | 93-104 | |
回收率
| The recovery of rat BMP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 89 | 84-93 | |
| EDTA plasma (n=4) | 93 | 88-99 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | pg/ml | OD1 | OD2 | Average | Corrected | | | 500 | 2.714 | 2.596 | 2.655 | 2.534 | | | 250 | 2.212 | 2.142 | 2.177 | 2.056 | | | 125 | 1.455 | 1.524 | 1.490 | 1.369 | | | 62.5 | 0.800 | 0.789 | 0.795 | 0.674 | | | 31.2 | 0.448 | 0.421 | 0.435 | 0.314 | | | 15.6 | 0.253 | 0.243 | 0.248 | 0.127 | | | 7.8 | 0.172 | 0.185 | 0.179 | 0.058 | | | 0 | 0.123 | 0.118 | 0.121 | | |
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货期
3-5 working days
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