产品描述
APOE是一种载脂蛋白,可以帮助调节胆固醇和三酰甘油等脂质分子在人体内的运输。APOE基因有三种主要的基因型,不同的基因型会影响人体对脂质代谢的调节能力。APOE还参与多种生理过程,如神经元的生长和修复、调节免疫反应等。
APOE基因型与多种疾病的发生风险有关,尤其是与神经系统相关的疾病,如阿尔茨海默病、帕金森病和多发性硬化等。APOEε4基因型是阿尔茨海默病的一个重要风险因素,而APOEε2基因型则被认为与心血管疾病的发生风险降低有关。因此,APOE对疾病的预防和发生机制的研究具有重要意义。
华美生物所提供的Mouse apolipoprotein E (Apo-E) ELISA Kit属于ELISA检测试剂盒,采用竞争法定量检测鼠血清、血浆、细胞培养上清、组织匀浆样本中的APOE,其灵敏度为1.56 ng/mL,检测范围为6.25 ng/mL-400 ng/mL。
别名
ApoeApolipoprotein E ELISA kit; Apo-E ELISA kit
缩写
APOE
Uniprot No
P08226
灵敏度
1.56 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse Apo-E in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:100 |
Average % |
99 |
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| Range % |
96-101 |
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| 1:200 |
Average % |
85 |
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| Range % |
81-89 |
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| 1:400 |
Average % |
100 |
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| Range % |
97-103 |
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| 1:800 |
Average % |
83 |
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| Range % |
80-86 |
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回收率
| The recovery of mouse Apo-E spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
97 |
93-100 |
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| EDTA plasma (n=4) |
99 |
95-104 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
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| 400 |
0.157 |
0.159 |
0.158 |
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| 200 |
0.249 |
0.269 |
0.259 |
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| 100 |
0.432 |
0.441 |
0.437 |
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| 50 |
0.643 |
0.672 |
0.658 |
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| 25 |
0.955 |
0.984 |
0.970 |
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| 12.5 |
1.199 |
1.172 |
1.186 |
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| 6.25 |
1.568 |
1.572 |
1.570 |
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| 0 |
2.573 |
2.591 |
2.582 |
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货期
3-5 working days