产品描述
This Human 5HT ELISA Kit was designed for the quantitative measurement of Human 5HT protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 4 ng/mL-800 ng/mL and the sensitivity is 2 ng/mL.
缩写
5HT
灵敏度
2 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Neuroscience
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<15% |
|
|
|
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV%<15% |
|
|
|
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
| |
|
|
|
|
|
|
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human 5-HT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
96 |
|
| Range % |
92-102 |
|
| 1:2 |
Average % |
94 |
|
| Range % |
91-98 |
|
| 1:4 |
Average % |
85 |
|
| Range % |
80-89 |
|
| 1:8 |
Average % |
95 |
|
| Range % |
89-99 |
|
回收率
| The recovery of human 5-HT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
95 |
90-99 |
|
| EDTA plasma (n=4) |
89 |
85-96 |
|
| |
|
|
|
|
|
|
| |
|
|
|
|
|
|
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
|
|
| 800 |
0.215 |
0.216 |
0.216 |
|
|
| 200 |
0.383 |
0.391 |
0.387 |
|
|
| 64 |
0.575 |
0.567 |
0.571 |
|
|
| 16 |
0.986 |
0.974 |
0.980 |
|
|
| 4 |
1.571 |
1.472 |
1.522 |
|
|
| 0 |
2.444 |
2.345 |
2.395 |
|
|
|
货期
3-5 working days