产品描述
This Human CASP9 ELISA Kit was designed for the quantitative measurement of Human CASP9 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.78 ng/mL-50 ng/mL and the sensitivity is 0.195 ng/mL.
别名
APAF-3 ELISA Kit; Apoptotic protease Mch-6 ELISA Kit; Apoptotic protease-activating factor 3 ELISA Kit; CASP-9 ELISA Kit; CASP9 ELISA Kit; CASP9_HUMAN ELISA Kit; Caspase 9 ELISA Kit; Caspase-9 subunit p10 ELISA Kit; ICE-LAP6 ELISA Kit; ICE-like apoptotic protease 6 ELISA Kit
缩写
CASP9
Uniprot No
P55211
灵敏度
0.195 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cell Biology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human Casp-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 90 | |
| Range % | 84-94 | |
| 1:2 | Average % | 96 | |
| Range % | 91-99 | |
| 1:4 | Average % | 91 | |
| Range % | 87-94 | |
| 1:8 | Average % | 100 | |
| Range % | 94-103 | |
回收率
| The recovery of human Casp-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 88-97 | |
| EDTA plasma (n=4) | 105 | 97-108 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 50 | 2.702 | 2.546 | 2.624 | 2.520 | | | 25 | 1.526 | 1.533 | 1.530 | 1.426 | | | 12.5 | 0.745 | 0.764 | 0.755 | 0.651 | | | 6.25 | 0.479 | 0.486 | 0.483 | 0.379 | | | 3.12 | 0.289 | 0.293 | 0.291 | 0.187 | | | 1.56 | 0.212 | 0.215 | 0.214 | 0.110 | | | 0.78 | 0.161 | 0.164 | 0.163 | 0.059 | | | 0 | 0.106 | 0.102 | 0.104 | | |
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货期
3-5 working days