产品描述

The Human alanyl (membrane) aminopeptidase (ANPEP) ELISA kit is a reliable and efficient tool for the quantitative detection of Aminopeptidase N (AP-N), also known as hAPN or CD13, in serum, plasma, and tissue homogenates from Homo sapiens (Human) samples.

The ANPEP ELISA kit offers a broad detection range of 31.25 pg/mL to 2000 pg/mL and an impressive sensitivity of 7.81 pg/mL, enabling accurate and precise quantification of AP-N levels in your samples. The assay time is short, ranging from 1 to 5 hours, and requires only a small sample volume of 50-100ul.

The assay principle is a sandwich method, where the AP-N in the sample is captured by a coated antibody and detected by a biotinylated detection antibody, followed by a streptavidin-HRP conjugate, and finally, substrate solution. The signal is detected at a wavelength of 450 nm.

别名

Alanyl (membrane) aminopeptidase ELISA Kit; Alanyl aminopeptidase ELISA Kit; Aminopeptidase M ELISA Kit; Aminopeptidase N ELISA Kit; AMPN_HUMAN ELISA Kit; ANPEP ELISA Kit; AP M ELISA Kit; AP N ELISA Kit; AP-M ELISA Kit; AP-N ELISA Kit; APN ELISA Kit; CD 13 ELISA Kit; CD13 ELISA Kit; CD13 antigen ELISA Kit; gp150 ELISA Kit; hAPN ELISA Kit; LAP 1 ELISA Kit; LAP1 ELISA Kit; Microsomal aminopeptidase ELISA Kit; Myeloid plasma membrane glycoprotein CD13 ELISA Kit; p150 ELISA Kit; PEPN ELISA Kit

缩写

ANPEP

Uniprot No

P15144

灵敏度

7.81 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cardiovascular

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human ANPEP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:20		Average %		91
		Range %		84-99
1:40		Average %		91
		Range %		85-96
1:80		Average %		96
		Range %		92-103
1:160		Average %		103
		Range %		96-109

回收率

The recovery of human ANPEP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		92		88-97
EDTA plasma (n=4)		96		90-102

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 2000 2.627 2.645 2.636 2.545 1000 2.131 2.298 2.215 2.124 500 1.458 1.502 1.480 1.389 250 0.731 0.755 0.743 0.652 125 0.377 0.352 0.365 0.274 62.5 0.201 0.212 0.207 0.116 31.25 0.189 0.188 0.189 0.098 0 0.087 0.094 0.091

货期

3-5 working days