产品描述
This Human CK-MB ELISA Kit was designed for the quantitative measurement of Human CK-MB protein in serum, plasma, urine, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.9 ng/mL-60 ng/mL and the sensitivity is 0.22 ng/mL.
别名
N/A
缩写
CK-MB
灵敏度
0.22 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human CK-MB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 87 | | |
| Range % | 84-90 | | |
| 1:2 | Average % | 104 | | |
| Range % | 101-107 | | |
| 1:4 | Average % | 98 | | |
| Range % | 94-102 | | |
| 1:8 | Average % | 101 | | |
| Range % | 98-104 | | |
回收率
| The recovery of human CK-MB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 85 | 81-89 | | |
| EDTA plasma (n=4) | 109 | 104-113 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | | 60 | 2.016 | 2.106 | 2.061 | 1.907 | | | | 30 | 1.646 | 1.604 | 1.625 | 1.471 | | | | 15 | 1.215 | 1.253 | 1.234 | 1.080 | | | | 7.5 | 0.859 | 0.848 | 0.854 | 0.700 | | | | 3.75 | 0.586 | 0.552 | 0.569 | 0.415 | | | | 1.8 | 0.370 | 0.367 | 0.369 | 0.215 | | | | 0.9 | 0.284 | 0.278 | 0.281 | 0.127 | | | | 0 | 0.155 | 0.153 | 0.154 | | | |
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货期
3-5 working days