产品描述

The product CSB-E09787h is a ready-to-use microwell, strip plate ELISA Kit for quantitatively detecting the amount of the Calreticulin (CRT/CALR) in human serum, plasma, urine, or tissue homogenates. This assay kit was designed and optimized for Tags & Cell Markers-related research in humans. It has undergone rigorous quality control in multiple parameters, including sensitivity, specificity, precision, linearity, recovery, and inter-batch difference. Refer to the product instructions for more details. This assay employs the quantitative sandwich enzyme immunoassay technique, in which CRT in the samples or standards are sandwiched between pre-coated CRT antibody and Biotin-conjugated CRT antibody. HRP-avidin is then added into the wells. Following a wash to remove any unbound reagent, the TMB substrate solution is added to the wells and color develops in proportion to the amount of CRT bound in the initial step. The color development is stopped upon adding the stop solution, and the intensity of the color is measured at 450 nm via a microplate reader. The levels of CRT in the samples can be determined by referring to the O.D. (optical density) of the samples to the standard curve.

CRT, also called CALR, is an endoplasmic reticulum (ER) chaperone protein involved in conformation-dependent molecular sorting of newly synthesized proteins (known as quality control (QC)). Within the ER, CALR normally binds misfolded proteins and retains them for ER-associated degradation (ERAD). Outside of the ER, CALR also participates in a variety of biological processes, including antigen processing and presentation for the adaptive immune response, cell adhesion/migration, cell proliferation, and immunogenic cell death. In the nucleus, CALR regulates gene expression and influences cell proliferation by suppressing interactions between retinoic acid receptor and its DNA response elements.

别名

Autoantigen RO ELISA Kit; CALR ELISA Kit; CALR protein ELISA Kit; CALR_HUMAN ELISA Kit; Calregulin ELISA Kit; Calreticulin ELISA Kit; cC1qR ELISA Kit; CRP55 ELISA Kit; CRT ELISA Kit; CRTC ELISA Kit; Endoplasmic reticulum resident protein 60 ELISA Kit; Epididymis secretory sperm binding protein Li 99n ELISA Kit; ERp60 ELISA Kit; FLJ26680 ELISA Kit; grp60 ELISA Kit; HACBP ELISA Kit; HEL S 99n ELISA Kit; RO ELISA Kit; Sicca syndrome antigen A (autoantigen Ro; calreticulin) ELISA Kit; Sicca syndrome antigen A ELISA Kit; SSA ELISA Kit

缩写

CALR

Uniprot No

P27797

灵敏度

0.039 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Tags & Cell Markers

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human CRT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		97
		Range %		92-101
1:2		Average %		93
		Range %		89-98
1:4		Average %		90
		Range %		85-95
1:8		Average %		104
		Range %		100-110

回收率

The recovery of human CRT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		84		80-89
EDTA plasma (n=4)		89		84-96

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 10 2.608 2.572 2.590 2.370 5 2.172 2.072 2.122 1.902 2.5 1.511 1.563 1.537 1.317 1.25 1.106 1.093 1.100 0.880 0.625 0.633 0.597 0.615 0.395 0.312 0.429 0.464 0.447 0.227 0.156 0.322 0.310 0.316 0.096 0 0.221 0.219 0.220

货期

3-5 working days