产品描述
The ELISA Kit is designed for quantitatively measuring mouse ADAM8 levels in samples, including serum, plasma, or tissue homogenates. It uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of ADAM8 in samples. The color intensity is measured at 450 nm via a microplate reader.
ADAM8 is a proteolytically active member of the ADAM protease family, which contains disintegrin and metalloprotease domains. The dysregulation of ADAM8 influences pathological conditions such as rheumatoid arthritis, asthma, and cancers. Abnormal expression of ADAM8 has been found in solid tumors, such as gliomas, lung cancer, pancreatic cancer, renal cell carcinomas, and prostate carcinomas. ADAM8 overexpression has been related to poor prognosis in hepatocellular carcinoma, breast cancer, and pancreatic adenocarcinoma, and might act as a potential therapeutic target. ADAM8 is implicated in tumorigenesis by stimulating angiogenesis, increasing cellular abilities of invasion and migration, and inhibiting cancer cell apoptosis.
别名
Adam8 ELISA kit; Ms2Disintegrin and metalloproteinase domain-containing protein 8 ELISA kit; ADAM 8 ELISA kit; EC 3.4.24.- ELISA kit; Cell surface antigen MS2 ELISA kit; Macrophage cysteine-rich glycoprotein ELISA kit; CD antigen CD156a ELISA kit
缩写
ADAM8
Uniprot No
Q05910
灵敏度
15.6 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse ADAM8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 91 | | |
| Range % | 86-98 | | |
| 1:2 | Average % | 97 | | |
| Range % | 92-107 | | |
| 1:4 | Average % | 93 | | |
| Range % | 87-98 | | |
| 1:8 | Average % | 87 | | |
| Range % | 82-94 | | |
回收率
| The recovery of mouse ADAM8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 92 | 87-97 | | |
| EDTA plasma (n=4) | 105 | 95-110 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
|
 | pg/ml | OD1 | OD2 | Average | Corrected | | | | 4000 | 2.226 | 2.344 | 2.285 | 2.171 | | | | 2000 | 1.806 | 1.923 | 1.865 | 1.751 | | | | 1000 | 1.341 | 1.354 | 1.348 | 1.234 | | | | 500 | 0.723 | 0.750 | 0.737 | 0.623 | | | | 250 | 0.440 | 0.411 | 0.426 | 0.312 | | | | 125 | 0.278 | 0.287 | 0.283 | 0.169 | | | | 62.5 | 0.213 | 0.205 | 0.209 | 0.095 | | | | 0 | 0.112 | 0.115 | 0.114 | | | |
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货期
3-5 working days
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