产品描述
This Human C-MAPT/C-TAU ELISA Kit was designed for the quantitative measurement of Human C-MAPT/C-TAU protein in serum, plasma, cerebrospinal fluid (CSF), tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.
别名
cleaved form of DDPAC, FLJ31424, FTDP-17, MAPTL, MGC138549, MSTD, MTBT1, MTBT2, PPND, TAU, G protein beta1/gamma2 subunit-interacting factor 1|microtubule-associated protein tau, isoform 4
缩写
C-MAPT/C-TAU
灵敏度
15.6 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human C-MAPT/C-TAU in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:5 | Average % | 102 | |
| Range % | 96-106 | |
| 1:10 | Average % | 100 | |
| Range % | 90-104 | |
| 1:20 | Average % | 95 | |
| Range % | 85-99 | |
| 1:40 | Average % | 94 | |
| Range % | 88-99 | |
回收率
| The recovery of human C-MAPT/C-TAU spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 90 | 82-95 | |
| EDTA plasma (n=4) | 100 | 91-104 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 4000 | 3.001 | 2.889 | 2.945 | 2.832 | | | 2000 | 2.114 | 1.976 | 2.045 | 1.932 | | | 1000 | 1.231 | 1.200 | 1.216 | 1.103 | | | 500 | 0.596 | 0.565 | 0.581 | 0.468 | | | 250 | 0.353 | 0.341 | 0.347 | 0.234 | | | 125 | 0.239 | 0.232 | 0.236 | 0.123 | | | 62.5 | 0.171 | 0.168 | 0.170 | 0.057 | | | 0 | 0.111 | 0.115 | 0.113 | | |
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货期
3-5 working days