产品描述

This Human CA2 ELISA Kit was designed for the quantitative measurement of Human CA2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

别名

CA 2 ELISA Kit; CA II ELISA Kit; CA-II ELISA Kit; Ca2 ELISA Kit; CAC ELISA Kit; CAH2_HUMAN ELISA Kit; CAII ELISA Kit; Car 2 ELISA Kit; Car2 ELISA Kit; Carbonate dehydratase II ELISA Kit; Carbonic anhydrase 2 ELISA Kit; Carbonic anhydrase B ELISA Kit; Carbonic anhydrase C ELISA Kit; Carbonic anhydrase C; formerly ELISA Kit; Carbonic anhydrase II ELISA Kit; Carbonic dehydratase ELISA Kit; epididymis luminal protein 76 ELISA Kit; Epididymis secretory protein Li 282 ELISA Kit; HEL-76 ELISA Kit; HEL-S-282 ELISA Kit

缩写

CA2

Uniprot No

P00918

灵敏度

0.078 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cell Biology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human CA-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		99
		Range %		92-103
1:2		Average %		102
		Range %		95-105
1:4		Average %		91
		Range %		85-94
1:8		Average %		88
		Range %		84-92

回收率

The recovery of human CA-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		90		85-94
EDTA plasma (n=4)		91		82-94

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected   20 2.700 2.542 2.621 2.502   10 2.191 2.113 2.152 2.033   5 1.374 1.351 1.363 1.244   2.5 0.765 0.754 0.760 0.641   1.25 0.373 0.379 0.376 0.257   0.625 0.310 0.323 0.317 0.198   0.312 0.215 0.211 0.213 0.094   0 0.120 0.118 0.119

货期

3-5 working days