产品描述
This Dog CK-MB ELISA Kit was designed for the quantitative measurement of Dog CK-MB protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 1.56 μIU/mL-100 μIU/mL and the sensitivity is 0.78 μIU/mL.
缩写
CK-MB
灵敏度
0.78 μIU/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of canine CK-MB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:100 | Average % | 84 | | |
| Range % | 80-88 | | |
| 1:200 | Average % | 98 | | |
| Range % | 94-102 | | |
| 1:400 | Average % | 90 | | |
| Range % | 86-94 | | |
| 1:800 | Average % | 92 | | |
| Range % | 88-96 | | |
回收率
| The recovery of canine CK-MB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 100 | 95-107 | | |
| EDTA plasma (n=4) | 89 | 85-93 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
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| μIU/ml | OD1 | OD2 | Average | | | | | 100 | 0.157 | 0.162 | 0.160 | | | | | 50 | 0.301 | 0.284 | 0.293 | | | | | 25 | 0.464 | 0.491 | 0.478 | | | | | 12.5 | 0.736 | 0.715 | 0.726 | | | | | 6.25 | 0.982 | 0.959 | 0.971 | | | | | 3.12 | 1.275 | 1.307 | 1.291 | | | | | 1.56 | 1.513 | 1.563 | 1.538 | | | | | 0 | 2.029 | 2.126 | 2.078 | | | |
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货期
3-5 working days