产品描述

This Human BRAF ELISA Kit was designed for the quantitative measurement of Human BRAF protein in serum, plasma, cell lysates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.

别名

FLJ95109 ELISA Kit; 94 kDa B raf protein ELISA Kit; B raf 1 ELISA Kit; B raf ELISA Kit; B Raf proto oncogene serine threonine protein kinase ELISA Kit; B Raf proto oncogene; serine/threonine kinase ELISA Kit; B RAF1 ELISA Kit; B-Raf proto-oncogene serine/threonine-protein kinase (p94) ELISA Kit; BRAF 1 ELISA Kit; BRAF ELISA Kit; BRAF_HUMAN ELISA Kit; BRAF1 ELISA Kit; cRmil ELISA Kit; MGC126806 ELISA Kit; MGC138284 ELISA Kit; Murine sarcoma viral (v-raf) oncogene homolog B1 ELISA Kit; Murine sarcoma viral v raf oncogene homolog B1 ELISA Kit; NS7 ELISA Kit; Oncogen BRAF ELISA Kit; oncogene BRAF1 ELISA Kit; p94 ELISA Kit; Proto-oncogene B-Raf ELISA Kit; Proto-oncogene c-Rmil ELISA Kit; RAFB 1 ELISA Kit; RAFB1 ELISA Kit; RMIL ELISA Kit; Serine/threonine-protein kinase B-raf ELISA Kit; v raf murine sarcoma viral oncogene homolog B ELISA Kit; v raf murine sarcoma viral oncogene homolog B1 ELISA Kit; v-Raf murine sarcoma viral oncogene homolog B1 ELISA Kit

缩写

BRAF

Uniprot No

P15056

灵敏度

7.8 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cell Biology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human BRAF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		90
		Range %		80-100
1:2		Average %		97
		Range %		91-105
1:4		Average %		99
		Range %		92-110
1:8		Average %		92
		Range %		86-98

回收率

The recovery of human BRAF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		95		89-104
EDTA plasma (n=4)		97		90-100

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 2000 2.408 2.432 2.420 2.279 1000 1.659 1.676 1.668 1.527 500 1.042 1.004 1.023 0.882 250 0.660 0.639 0.650 0.509 125 0.429 0.424 0.427 0.286 62.5 0.289 0.275 0.282 0.141 31.25 0.214 0.229 0.222 0.081 0 0.145 0.137 0.141

货期

3-5 working days