产品描述
肿瘤坏死因子 α 转换酶 (TACE) 是一种锌金属蛋白酶,在调节肿瘤坏死因子 (TNF) 信号传导中起关键作用。它参与将膜结合的 TNFα 裂解成可溶性形式,然后可与细胞表面受体结合并激活下游通路。 TACE 与多种疾病有关,例如癌症、炎症和自身免疫性疾病。 FILTER 是一种 TACE 抑制剂,已被证明可以减少炎症并提高癌症治疗的疗效。
华美生物所提供的Human TNF α converting enzyme,TACE ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测人血清、血浆、组织匀浆、细胞裂解液样本中的ADAM17,其灵敏度为华美生物所提供的15.6 pg/mL,检测范围为62.5 pg/mL-4000 pg/mL。
别名
ADAM17; CSVP; TACE; Disintegrin and metalloproteinase domain-containing protein 17; ADAM 17; Snake venom-like protease; TNF-alpha convertase; TNF-alpha-converting enzyme; CD antigen CD156b
缩写
ADAM17
Uniprot No
P78536
灵敏度
15.6 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human TACE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
84 |
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| Range % |
80-90 |
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| 1:2 |
Average % |
97 |
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| Range % |
92-103 |
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| 1:4 |
Average % |
92 |
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| Range % |
86-99 |
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| 1:8 |
Average % |
93 |
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| Range % |
85-98 |
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回收率
| The recovery of human TACE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
89-100 |
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| EDTA plasma (n=4) |
97 |
90-103 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 4000 |
1.931 |
1.872 |
1.902 |
1.770 |
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| 2000 |
1.648 |
1.596 |
1.622 |
1.490 |
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| 1000 |
1.335 |
1.304 |
1.320 |
1.188 |
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| 500 |
1.082 |
1.012 |
1.047 |
0.915 |
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| 250 |
0.643 |
0.609 |
0.626 |
0.494 |
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| 125 |
0.409 |
0.379 |
0.394 |
0.262 |
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| 62.5 |
0.274 |
0.257 |
0.266 |
0.134 |
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| 0 |
0.135 |
0.128 |
0.132 |
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货期
3-5 working days