产品描述
APOB全称为载脂蛋白B(Apolipoprotein B)。它是胆固醇和三酰甘油的主要载体之一,可以结合和运输这些脂质分子到全身的细胞中。
APOB有两种形式,APOB-48和APOB-100。APOB-48主要存在于肠道细胞中,而APOB-100则主要存在于肝细胞中。这两种形式的差异在于它们的长度,APOB-48是APOB-100的N端片段,长度为48%。
APOB在血液中的含量可以作为血脂代谢异常的指标。高水平的APOB与动脉粥样硬化和心血管疾病的风险增加相关联。因此,测量APOB水平可以用于评估心血管疾病的风险,并指导相关治疗措施的制定。
华美生物所提供的Mouse Apolipoprotein B(APOB) ELISA kit属于ELISA检测试剂盒,采用竞争法定量检测鼠血清、血浆、组织匀浆样本中的APOB,其灵敏度为0.039 μg/mL,检测范围为0.156 μg/mL-10 μg/mL。
别名
ApobApolipoprotein B-100 ELISA kit; Apo B-100) [Cleaved into: Apolipoprotein B-48 ELISA kit; Apo B-48)] ELISA kit
缩写
APOB
Uniprot No
E9Q414
灵敏度
0.039 μg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse APOB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:100 |
Average % |
102 |
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| Range % |
97-108 |
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| 1:200 |
Average % |
103 |
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| Range % |
97-106 |
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| 1:400 |
Average % |
92 |
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| Range % |
88-97 |
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| 1:800 |
Average % |
86 |
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| Range % |
82-91 |
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回收率
| The recovery of mouse APOB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
94 |
89-98 |
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| EDTA plasma (n=4) |
105 |
102-109 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| μg/ml |
OD1 |
OD2 |
Average |
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| 10 |
0.091 |
0.092 |
0.092 |
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| 5 |
0.136 |
0.132 |
0.134 |
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| 2.5 |
0.236 |
0.229 |
0.233 |
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| 1.25 |
0.360 |
0.368 |
0.364 |
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| 0.625 |
0.539 |
0.524 |
0.532 |
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| 0.312 |
0.877 |
0.927 |
0.902 |
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| 0.156 |
1.334 |
1.286 |
1.310 |
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| 0 |
2.562 |
2.747 |
2.655 |
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货期
3-5 working days