产品描述
This Human Aβ1-40 ELISA Kit was designed for the quantitative measurement of Human Aβ1-40 protein in serum, tissue homogenates, cerebrospinal fluid (CSF). It is a Sandwich ELISA kit, its detection range is 125 pg/mL-8000 pg/mL and the sensitivity is 31.25 pg/mL.
别名
amyloid beta peptide 1-40,Aβ1-40,amyloid β peptide 1-40
缩写
Aβ1-40
灵敏度
31.25 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Neuroscience
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human Aβ1-40 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 87 | |
| Range % | 80-92 | |
| 1:2 | Average % | 99 | |
| Range % | 91-105 | |
| 1:4 | Average % | 95 | |
| Range % | 92-98 | |
| 1:8 | Average % | 103 | |
| Range % | 96-110 | |
回收率
| The recovery of human Aβ1-40 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 98 | 89-100 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 8000 | 2.846 | 2.687 | 2.767 | 2.610 | | | 4000 | 2.237 | 2.089 | 2.163 | 2.006 | | | 2000 | 1.679 | 1.612 | 1.646 | 1.489 | | | 1000 | 1.246 | 1.211 | 1.229 | 1.072 | | | 500 | 0.736 | 0.697 | 0.717 | 0.560 | | | 250 | 0.512 | 0.498 | 0.505 | 0.348 | | | 125 | 0.303 | 0.296 | 0.300 | 0.143 | | | 0 | 0.160 | 0.154 | 0.157 | | |
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货期
3-5 working days