产品描述

This Human CD274 ELISA Kit was designed for the quantitative measurement of Human CD274 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL .

别名

B7 H ELISA Kit; B7 H1 ELISA Kit; B7 homolog 1 ELISA Kit; B7-H1 ELISA Kit; B7H ELISA Kit; B7H1 ELISA Kit; CD 274 ELISA Kit; CD274 ELISA Kit; CD274 antigen ELISA Kit; CD274 molecule ELISA Kit; MGC142294 ELISA Kit; MGC142296 ELISA Kit; OTTHUMP00000021029 ELISA Kit; PD L1 ELISA Kit; PD-L1 ELISA Kit; PD1L1_HUMAN ELISA Kit; PDCD1 ligand 1 ELISA Kit; PDCD1L1 ELISA Kit; PDCD1LG1 ELISA Kit; PDL 1 ELISA Kit; PDL1 ELISA Kit; Programmed cell death 1 ligand 1 ELISA Kit; Programmed death ligand 1 ELISA Kit; RGD1566211 ELISA Kit

缩写

CD274

Uniprot No

Q9NZQ7

灵敏度

3.9 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Others

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human PD-L1/CD274 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		90
		Range %		80-101
1:2		Average %		95
		Range %		91-105
1:4		Average %		98
		Range %		90-107
1:8		Average %		95
		Range %		85-100

回收率

The recovery of human PD-L1/CD274 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		96		89-104
EDTA plasma (n=4)		95		90-107

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 1000 2.274 2.174 2.224 1.965 500 1.940 1.854 1.897 1.638 250 1.450 1.355 1.403 1.144 125 0.927 0.955 0.941 0.682 62.5 0.570 0.580 0.575 0.316 31.2 0.422 0.417 0.420 0.161 15.6 0.352 0.341 0.347 0.088 0 0.253 0.265 0.259

货期

3-5 working days