产品描述
This Human CTSD ELISA Kit was designed for the quantitative measurement of Human CTSD protein in serum, plasma, tissue homogenates, ascitic fluid. It is a Sandwich ELISA kit, its detection range is 83 pg/mL-2000 pg/mL and the sensitivity is 33 pg/mL .
别名
CatD ELISA Kit; CATD_HUMAN ELISA Kit; Cathepsin D ELISA Kit; Cathepsin D heavy chain ELISA Kit; CD ELISA Kit; Ceroid lipofuscinosis neuronal 10 ELISA Kit; CLN10 ELISA Kit; CPSD ELISA Kit; ctsd ELISA Kit; Epididymis secretory sperm binding protein Li 130P ELISA Kit; HEL S 130P ELISA Kit; Lysosomal aspartyl peptidase ELISA Kit; Lysosomal aspartyl protease ELISA Kit; MGC2311 ELISA Kit
缩写
CTSD
Uniprot No
P07339
灵敏度
33 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<10% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<15% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human cath-D in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 92 | |
| Range % | 85-98 | |
| 1:2 | Average % | 96 | |
| Range % | 88-100 | |
| 1:4 | Average % | 95 | |
| Range % | 89-100 | |
| 1:8 | Average % | 97 | |
| Range % | 91-101 | |
回收率
| The recovery of human cath-D spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 92 | 89-96 | |
| EDTA plasma (n=4) | 95 | 91-101 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 2000 | 2.004 | 1.997 | 2.001 | 1.953 | | | 1000 | 1.321 | 1.356 | 1.339 | 1.291 | | | 500 | 0.785 | 0.821 | 0.803 | 0.755 | | | 167 | 0.364 | 0.341 | 0.353 | 0.305 | | | 83 | 0.216 | 0.229 | 0.223 | 0.175 | | | 0 | 0.047 | 0.048 | 0.048 | | |
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货期
3-5 working days