产品描述
The Rat Keratinocyte Autocrine Factor/Amphiregulin (KAF/AR) ELISA Kit is designed to measure Amphiregulin (AR) in rat serum, plasma, and tissue homogenates with exceptional accuracy and sensitivity.
With a detection range of 1.25 pg/mL-80 pg/mL and a sensitivity of 0.31 pg/mL, this quantitative assay can detect even the smallest amount of KAF/AR in your samples. The assay time is only 1-5 hours, and the required sample volume is just 50-100ul. The detection wavelength is 450 nm, ensuring reliable and precise results every time.
This kit is ideal for researchers investigating the role of KAF/AR in cancer、activates intracellular signaling, lung morphogenesis, breast bone tissue and oocyte maturation, and tissue integrity recovery after infection or injury（激活细胞内信号、肺形态发生及乳腺骨组织和卵母细胞的发育成熟、感染或损伤后的组织完整性恢复）. The sandwich measurement principle allows for accurate quantification of KAF/AR in your samples, making it a valuable tool for any cancer research project. With its quick assay time, low sample volume requirement, and precise results, this kit is a must-have for any researcher studying cancer.
别名
Areg; Sdgf; Amphiregulin; AR; Schwannoma-derived growth factor; SDGF
缩写
AREG
Uniprot No
P24338
灵敏度
0.31 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat KAF/AR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 92 | |
| Range % | 87-98 | |
| 1:2 | Average % | 95 | |
| Range % | 91-99 | |
| 1:4 | Average % | 86 | |
| Range % | 80-92 | |
| 1:8 | Average % | 96 | |
| Range % | 92-102 | |
回收率
| The recovery of rat KAF/AR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 104 | 98-108 | |
| EDTA plasma (n=4) | 97 | 92-106 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | pg/ml | OD1 | OD2 | Average | Corrected | | | 80 | 2.538 | 2.616 | 2.577 | 2.413 | | | 40 | 1.939 | 2.059 | 1.999 | 1.835 | | | 20 | 1.364 | 1.386 | 1.375 | 1.211 | | | 10 | 0.882 | 0.870 | 0.876 | 0.712 | | | 5 | 0.549 | 0.541 | 0.545 | 0.381 | | | 2.5 | 0.341 | 0.356 | 0.349 | 0.185 | | | 1.25 | 0.253 | 0.258 | 0.256 | 0.092 | | | 0 | 0.168 | 0.160 | 0.164 | | |
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货期
3-5 working days
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