产品描述
人ADAMDEC1 ELISA试剂盒是为了定量测量血清、血浆和组织匀浆中的人类ADAMDEC1蛋白而设计的。华美生物夹心ELISA试剂盒,其检测范围为62.5 pg/mL-4000 pg/mL,灵敏度为15.6 pg/mL。
别名
A disintegrin and metalloproteinase domain like protein decysin 1 ELISA Kit; A disintegrin and metalloproteinase domain-like protein decysin-1 ELISA Kit; ADAM DEC1 ELISA Kit; ADAM like decysin 1 ELISA Kit; ADAM like protein decysin 1 ELISA Kit; ADAM-like protein decysin-1 ELISA Kit; ADAMDEC 1 ELISA Kit; Adamdec1 ELISA Kit; ADEC1_HUMAN ELISA Kit; Decysin ELISA Kit; Disintegrin protease ELISA Kit; M12.219 ELISA Kit
缩写
ADAMDEC1
Uniprot No
O15204
灵敏度
15.6 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cell Biology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human ADAMDEC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
92 |
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| Range % |
87-96 |
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| 1:2 |
Average % |
96 |
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| Range % |
90-101 |
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| 1:4 |
Average % |
94 |
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| Range % |
89-99 |
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| 1:8 |
Average % |
97 |
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| Range % |
92-103 |
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回收率
| The recovery of human ADAMDEC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
104 |
99-108 |
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| EDTA plasma (n=4) |
94 |
89-98 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 4000 |
2.224 |
2.364 |
2.294 |
2.141 |
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| 2000 |
1.732 |
1.667 |
1.700 |
1.547 |
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| 1000 |
1.127 |
1.071 |
1.099 |
0.946 |
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| 500 |
0.702 |
0.713 |
0.708 |
0.555 |
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| 250 |
0.431 |
0.442 |
0.437 |
0.284 |
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| 125 |
0.314 |
0.321 |
0.318 |
0.165 |
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| 62.5 |
0.246 |
0.252 |
0.249 |
0.096 |
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| 0 |
0.152 |
0.154 |
0.153 |
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货期
3-5 working days