产品描述

This Human COL6A1 ELISA Kit was designed for the quantitative measurement of Human COL6A1 protein in serum, plasma, cell culture supernates, urine. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL .

别名

Alpha 1 (VI) chain (61 AA) ELISA Kit; CO6A1_HUMAN ELISA Kit; COL6A1 ELISA Kit; COL6A2 ELISA Kit; COL6A3 ELISA Kit; collagen 6 ELISA Kit; Collagen alpha 2(VI) chain ELISA Kit; Collagen alpha 3(VI) chain ELISA Kit; Collagen alpha-1(VI) chain ELISA Kit; collagen six ELISA Kit; Collagen type VI alpha 1 ELISA Kit; Collagen type VI alpha 2 ELISA Kit; Collagen type VI alpha 3 ELISA Kit; Collagen VI alpha 1 polypeptide ELISA Kit; Collagen VI alpha 2 polypeptide ELISA Kit; Collagen VI alpha 3 polypeptide ELISA Kit; CollagenVI ELISA Kit; Human mRNA for collagen VI alpha 2 C terminal globular domain ELISA Kit; OPLL ELISA Kit; PP3610 ELISA Kit

缩写

COL6A1

Uniprot No

P12109

灵敏度

7.81 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human COLVI in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:200		Average %		86
		Range %		80-96
1:400		Average %		97
		Range %		91-98
1:800		Average %		98
		Range %		92-105
1:1600		Average %		102
		Range %		86-110

回收率

The recovery ofhuman COLVI spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		98		89-110
EDTA plasma (n=4)		96		90-100

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 2000 2.912 2.812 2.862 2.702 1000 2.137 2.103 2.120 1.960 500 1.156 1.098 1.127 0.967 250 0.614 0.578 0.596 0.436 125 0.372 0.346 0.359 0.199 62.5 0.281 0.279 0.280 0.120 31.25 0.202 0.198 0.200 0.040 0 0.161 0.159 0.160

货期

3-5 working days