产品描述
This Rabbit Col ELISA Kit was designed for the quantitative measurement of Rabbit Col protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 6.25 ng/mL-400 ng/mL and the sensitivity is 1.56 ng/mL.
别名
Collagen Type 2,Col 2,_x000D_
Collagen Type II,Col II
缩写
Col
灵敏度
1.56 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit Col Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
98 |
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| Range % |
90-102 |
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| 1:2 |
Average % |
107 |
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| Range % |
97-111 |
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| 1:4 |
Average % |
88 |
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| Range % |
82-92 |
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| 1:8 |
Average % |
98 |
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| Range % |
91-102 |
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回收率
| The recovery of rabbit Col Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
90 |
82-94 |
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| EDTA plasma (n=4) |
102 |
92-107 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 400 |
2.576 |
2.528 |
2.552 |
2.457 |
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| 200 |
1.949 |
1.986 |
1.968 |
1.873 |
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| 100 |
1.421 |
1.366 |
1.394 |
1.299 |
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| 50 |
0.827 |
0.811 |
0.819 |
0.724 |
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| 25 |
0.434 |
0.417 |
0.426 |
0.331 |
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| 12.5 |
0.314 |
0.302 |
0.308 |
0.213 |
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| 6.25 |
0.234 |
0.221 |
0.228 |
0.133 |
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| 0 |
0.093 |
0.097 |
0.095 |
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货期
3-5 working days