产品描述
BCL2 (B-cell lymphoma 2)是一种重要的细胞凋亡调节因子,调节细胞内凋亡程序的启动。BCL2在体内分布广泛,包括淋巴细胞、神经元、内皮细胞、胰岛素细胞和卵巢等组织中。
华美生物所提供的Rat B-cell CLL/lymphoma 2 (BCL2) ELISA kit属于ELISA检测试剂盒,采用双抗夹心法定量检测大鼠血清、血浆、组织匀浆样本中的BCL2,其灵敏度为0.078 ng/mL,检测范围为0.312 ng/mL-20 ng/mL。
别名
Bcl2; Bcl-2; Apoptosis regulator Bcl-2
缩写
BCL2
Uniprot No
P49950
灵敏度
0.078 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cell Biology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat BCL2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
87 |
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| Range % |
80-95 |
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| 1:2 |
Average % |
95 |
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| Range % |
85-99 |
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| 1:4 |
Average % |
95 |
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| Range % |
86-102 |
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| 1:8 |
Average % |
98 |
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| Range % |
82-104 |
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回收率
| The recovery of rat BCL2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
85-100 |
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| EDTA plasma (n=4) |
95 |
87-102 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 20 |
2.745 |
2.768 |
2.757 |
2.572 |
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| 10 |
2.176 |
2.165 |
2.171 |
1.986 |
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| 5 |
1.485 |
1.463 |
1.474 |
1.289 |
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| 2.5 |
0.896 |
0.864 |
0.880 |
0.695 |
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| 1.25 |
0.569 |
0.543 |
0.556 |
0.371 |
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| 0.625 |
0.425 |
0.439 |
0.432 |
0.247 |
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| 0.312 |
0.296 |
0.284 |
0.290 |
0.105 |
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| 0 |
0.185 |
0.184 |
0.185 |
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货期
3-5 working days