产品描述
This Rat CCL3 ELISA Kit was designed for the quantitative measurement of Rat CCL3 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 12.5 pg/mL-800 pg/mL and the sensitivity is 3.12 pg/mL .
别名
Ccl3 ELISA Kit; Mip1a ELISA Kit; Scya3 ELISA Kit; C-C motif chemokine 3 ELISA Kit; Macrophage inflammatory protein 1-alpha ELISA Kit; MIP-1-alpha ELISA Kit; Small-inducible cytokine A3 ELISA Kit
缩写
CCL3
Uniprot No
P50229
灵敏度
3.12 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat MIP-1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 88 | |
| Range % | 84-98 | |
| 1:2 | Average % | 96 | |
| Range % | 91-104 | |
| 1:4 | Average % | 99 | |
| Range % | 96-105 | |
| 1:8 | Average % | 95 | |
| Range % | 89-99 | |
回收率
| The recovery of rat MIP-1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 104 | 99-108 | |
| EDTA plasma (n=4) | 95 | 91-104 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 800 | 2.152 | 2.051 | 2.102 | 1.969 | | | 400 | 1.484 | 1.383 | 1.434 | 1.301 | | | 200 | 0.888 | 0.867 | 0.878 | 0.745 | | | 100 | 0.522 | 0.511 | 0.517 | 0.384 | | | 50 | 0.336 | 0.347 | 0.342 | 0.209 | | | 25 | 0.253 | 0.264 | 0.259 | 0.126 | | | 12.5 | 0.195 | 0.199 | 0.197 | 0.064 | | | 0 | 0.132 | 0.133 | 0.133 | | |
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货期
3-5 working days