产品描述

This Human CD109 ELISA Kit was designed for the quantitative measurement of Human CD109 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 94 pg/mL-6000 pg/mL and the sensitivity is 23.4 pg/mL.

别名

150 KDa TGF beta 1 binding protein ELISA Kit; 150 KDa TGF-beta-1-binding protein ELISA Kit; 7D1 ELISA Kit; 8A3 ELISA Kit; Activated T cell marker CD109 ELISA Kit; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 7 ELISA Kit; CD 109 ELISA Kit; CD109 ELISA Kit; CD109 antigen ELISA Kit; CD109_HUMAN ELISA Kit; CPAMD7 ELISA Kit; DKFZp762L1111 ELISA Kit; E123 ELISA Kit; FLJ38569 ELISA Kit; FLJ41966 ELISA Kit; Gov system alloantigens on platelets ELISA Kit; p180 ELISA Kit; Platelet specific Gov antigen ELISA Kit; Platelet-specific Gov antigen ELISA Kit; r150 ELISA Kit; RP11 525G3.1 ELISA Kit

缩写

CD109

Uniprot No

Q6YHK3

灵敏度

23.4 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Immunology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human CD109 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		89
		Range %		85-92
1:2		Average %		84
		Range %		80-88
1:4		Average %		96
		Range %		93-99
1:8		Average %		102
		Range %		98-106

回收率

The recovery of human CD109 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		94		90-98
EDTA plasma (n=4)		85		81-89

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 6000 2.008 2.157 2.083 1.928 3000 1.604 1.630 1.617 1.462 1500 1.191 1.175 1.183 1.028 750 0.784 0.772 0.778 0.623 375 0.472 0.498 0.485 0.330 187.5 0.304 0.315 0.310 0.155 94 0.212 0.216 0.214 0.059 0 0.152 0.157 0.155

货期

3-5 working days