产品描述

This Human ARPC2 ELISA Kit was designed for the quantitative measurement of Human ARPC2 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 23.5 pg/mL-1500 pg/mL and the sensitivity is 5.8 pg/mL.

别名

Actin related protein 2/3 complex subunit 2 34kDa ELISA Kit; Actin related protein 2/3 complex subunit 2 ELISA Kit; Actin-related protein 2/3 complex subunit 2 ELISA Kit; ARC 34 ELISA Kit; ARC34 ELISA Kit; Arp2/3 complex 34 kDa subunit ELISA Kit; ARP2/3 protein compex subunit 34 ELISA Kit; ARPC 2 ELISA Kit; Arpc2 ELISA Kit; ARPC2_HUMAN ELISA Kit; p34 Arc ELISA Kit; p34-ARC ELISA Kit; p34Arc ELISA Kit; PNAS 139 ELISA Kit; PNAS139 ELISA Kit; PRO 2446 ELISA Kit; PRO2446 ELISA Kit

缩写

ARPC2

Uniprot No

O15144

灵敏度

5.8 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human ARPC2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		86
		Range %		80-92
1:2		Average %		95
		Range %		90-105
1:4		Average %		99
		Range %		92-110
1:8		Average %		91
		Range %		85-98

回收率

The recovery of human ARPC2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		92		87-97
EDTA plasma (n=4)		90		85-100

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 1500 2.201 2.122 2.162 2.011 750 1.564 1.476 1.520 1.369 375 1.007 0.953 0.980 0.829 187.5 0.683 0.634 0.659 0.508 94 0.492 0.453 0.473 0.322 47 0.354 0.331 0.343 0.192 23.5 0.246 0.239 0.243 0.092 0 0.155 0.146 0.151

货期

3-5 working days