产品描述

This Human CMA1 ELISA Kit was designed for the quantitative measurement of Human CMA1 protein in serum, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL .

别名

Alpha-chymase ELISA Kit; Chymase 1 ELISA Kit; Chymase 1 mast cell ELISA Kit; chymase 1 preproprotein transcript E ELISA Kit; chymase 1 preproprotein transcript I ELISA Kit; Chymase ELISA Kit; Chymase; heart ELISA Kit; Chymase; mast cell ELISA Kit; CMA1 ELISA Kit; CMA1_HUMAN ELISA Kit; CYH ELISA Kit; CYM ELISA Kit; EC 3.4.21.39 ELISA Kit; Mast cell chymase 1 ELISA Kit; Mast cell protease 3 ELISA Kit; Mast cell protease 5 ELISA Kit; Mast cell protease I ELISA Kit; Mast cell protease III ELISA Kit; Mcp-5 ELISA Kit; MCP3P ELISA Kit; Mcpt5 ELISA Kit; MCT1 ELISA Kit; MGC119890 ELISA Kit; MGC119891 ELISA Kit; MMCP-5 ELISA Kit

缩写

CMA1

Uniprot No

P23946

灵敏度

0.039 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cardiovascular

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human mast cell chymase (CMA1) in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		98
		Range %		94-103
1:2		Average %		90
		Range %		84-95
1:4		Average %		99
		Range %		94-104
1:8		Average %		89
		Range %		85-94

回收率

The recovery of human mast cell chymase(CMA1) spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		90		85-95
EDTA plasma (n=4)		101		95-107

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 10 2.735 2.789 2.762 2.656 5 2.397 2.385 2.391 2.285 2.5 1.832 1.869 1.851 1.745 1.25 1.256 1.241 1.249 1.143 0.625 0.725 0.756 0.741 0.635 0.312 0.453 0.468 0.461 0.355 0.156 0.289 0.281 0.285 0.179 0 0.105 0.107 0.106

货期

3-5 working days