产品描述
This Mouse BCL2 ELISA Kit was designed for the quantitative measurement of Mouse BCL2 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.
别名
Bcl2 ELISA Kit; Bcl-2 ELISA Kit; Apoptosis regulator Bcl-2 ELISA Kit
缩写
BCL2
Uniprot No
P10417
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cell Biology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse Bcl-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:5 |
Average % |
96 |
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| Range % |
85-102 |
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| 1:10 |
Average % |
94 |
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| Range % |
85-102 |
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| 1:20 |
Average % |
94 |
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| Range % |
82-103 |
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| 1:40 |
Average % |
90 |
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| Range % |
81-99 |
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回收率
| The recovery of mouse Bcl-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
94 |
85-100 |
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| EDTA plasma (n=4) |
96 |
87-105 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1000 |
1.952 |
2.052 |
2.002 |
1.838 |
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| 500 |
1.527 |
1.591 |
1.559 |
1.395 |
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| 250 |
1.051 |
1.112 |
1.082 |
0.918 |
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| 125 |
0.628 |
0.592 |
0.610 |
0.446 |
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| 62.5 |
0.435 |
0.457 |
0.446 |
0.282 |
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| 31.2 |
0.293 |
0.304 |
0.299 |
0.135 |
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| 15.6 |
0.215 |
0.206 |
0.211 |
0.047 |
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| 0 |
0.166 |
0.162 |
0.164 |
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货期
3-5 working days