产品描述
This Mouse CK-MM ELISA Kit was designed for the quantitative measurement of Mouse CK-MM protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 0.156 mU/mL-10 mU/mL and the sensitivity is 0.039 mU/mL.
缩写
CK-MM
灵敏度
0.039 mU/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Metabolism
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse CK-MM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 87 | |
| Range % | 83-98 | |
| 1:2 | Average % | 98 | |
| Range % | 92-103 | |
| 1:4 | Average % | 104 | |
| Range % | 98-108 | |
| 1:8 | Average % | 93 | |
| Range % | 87-99 | |
回收率
| The recovery of mouse CK-MM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 92 | 85-99 | |
| EDTA plasma (n=4) | 100 | 95-105 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| mU/ml | OD1 | OD2 | Average | Corrected | | | 10 | 2.420 | 2.566 | 2.493 | 2.343 | | | 5 | 1.708 | 1.855 | 1.782 | 1.632 | | | 2.5 | 0.970 | 1.002 | 0.986 | 0.836 | | | 1.25 | 0.556 | 0.601 | 0.579 | 0.429 | | | 0.625 | 0.359 | 0.369 | 0.364 | 0.214 | | | 0.312 | 0.270 | 0.278 | 0.274 | 0.124 | | | 0.156 | 0.197 | 0.194 | 0.196 | 0.046 | | | 0 | 0.155 | 0.145 | 0.150 | | |
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货期
3-5 working days