产品描述
This Mouse ANG-Ⅱ ELISA Kit was designed for the quantitative measurement of Mouse ANG-Ⅱ protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.45 pg/mL-30 pg/mL and the sensitivity is 0.11 pg/mL .
别名
angiotensin Ⅱ, angiotensin 2, ANG2
缩写
ANG-Ⅱ
灵敏度
0.11 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse ANG-Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 93 | |
| Range % | 90-95 | |
| 1:2 | Average % | 102 | |
| Range % | 98-104 | |
| 1:4 | Average % | 92 | |
| Range % | 89-96 | |
| 1:8 | Average % | 100 | |
| Range % | 95-103 | |
回收率
| The recovery of mouse ANG-Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 93 | 90-95 | |
| EDTA plasma (n=4) | 96 | 92-99 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 30 | 2.166 | 2.072 | 2.119 | 1.980 | | | 15 | 1.434 | 1.422 | 1.428 | 1.289 | | | 7.5 | 0.985 | 0.971 | 0.978 | 0.839 | | | 3.75 | 0.489 | 0.497 | 0.493 | 0.354 | | | 1.8 | 0.313 | 0.324 | 0.319 | 0.180 | | | 0.9 | 0.238 | 0.242 | 0.240 | 0.101 | | | 0.45 | 0.187 | 0.181 | 0.184 | 0.045 | | | 0 | 0.140 | 0.138 | 0.139 | | |
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货期
3-5 working days