产品描述

This Human BIRC2 ELISA Kit was designed for the quantitative measurement of Human BIRC2 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 23.5 pg/mL-1500 pg/mL and the sensitivity is 5.8 pg/mL.

别名

API 1 ELISA Kit; API1 ELISA Kit; Apoptosis inhibitor 1 ELISA Kit; Baculoviral IAP repeat containing 2 ELISA Kit; Baculoviral IAP repeat containing protein 2 ELISA Kit; Baculoviral IAP repeat-containing protein 2 ELISA Kit; BIRC 2 ELISA Kit; BIRC2 ELISA Kit; BIRC2_HUMAN ELISA Kit; C IAP1 ELISA Kit; C-IAP1 ELISA Kit; Cellular inhibitor of apoptosis 1 ELISA Kit; cellular inhibitor of apoptosis protein 1 ELISA Kit; cIAP 1 ELISA Kit; cIAP1 ELISA Kit; HIAP 2 ELISA Kit; HIAP-2 ELISA Kit; HIAP2 ELISA Kit; IAP 2 ELISA Kit; IAP homolog B ELISA Kit; IAP-2 ELISA Kit; IAP2 ELISA Kit; Inhibitor of apoptosis protein 2 ELISA Kit; MIHB ELISA Kit; NFR2 TRAF signalling complex protein ELISA Kit; RING finger protein 48 ELISA Kit; RNF 48 ELISA Kit; RNF48 ELISA Kit; TNFR2 TRAF signaling complex protein 2 ELISA Kit; TNFR2-TRAF-signaling complex protein 2 ELISA Kit

缩写

BIRC2

Uniprot No

Q13490

灵敏度

5.8 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cell Biology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human BIRC2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		93
		Range %		83-97
1:2		Average %		86
		Range %		81-92
1:4		Average %		94
		Range %		897-98
1:8		Average %		103
		Range %		96-107

回收率

The recovery of human BIRC2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		89		82-94
EDTA plasma (n=4)		101		97-106

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected 1500 2.096 2.113 2.105 1.947 750 1.278 1.387 1.333 1.175 375 0.839 0.867 0.853 0.695 187.5 0.556 0.515 0.536 0.378 94 0.340 0.348 0.344 0.186 47 0.277 0.287 0.282 0.124 23.5 0.225 0.231 0.228 0.070 0 0.157 0.158 0.158

货期

3-5 working days