产品描述
CSTB是一种半胱氨酸蛋白酶抑制剂,主要在脑部神经元和其他组织中发现。CSTB与许多神经疾病的发病机制有关,如癫痫和阿尔茨海默病等。CSTB还可以影响细胞凋亡和炎症反应等方面的生物学过程。
华美生物所提供的Human Cystatin-B (CSTB/CST6/STFB) ELISA kit属于ELISA检测试剂盒,采用双抗夹心法定量检测人血清、血浆、细胞培养上清、尿液、唾液、组织匀浆、细胞裂解液样本中的CSTB,其灵敏度为0.233 ng/mL,检测范围为0.313 ng/mL-20 ng/mL。
别名
CHROW21 ELISA Kit; CPI B ELISA Kit; CPI-B ELISA Kit; CST 6 ELISA Kit; CST6 ELISA Kit; CSTB ELISA Kit; Cystatin B (stefin B) ELISA Kit; Cystatin B ELISA Kit; Cystatin-B ELISA Kit; CYTB ELISA Kit; CYTB_HUMAN ELISA Kit; EPM1 ELISA Kit; EPM1A ELISA Kit; Liver thiol proteinase inhibitor ELISA Kit; PME ELISA Kit; Stefin-B ELISA Kit; STF B ELISA Kit; STFB ELISA Kit; ULD ELISA Kit
缩写
CSTB
Uniprot No
P04080
灵敏度
0.233 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human CSTB/CST6/STFB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
104 |
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| Range % |
101-106 |
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| 1:2 |
Average % |
91 |
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| Range % |
88-97 |
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| 1:4 |
Average % |
96 |
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| Range % |
91-104 |
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| 1:8 |
Average % |
89 |
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| Range % |
84-94 |
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回收率
| The recovery of human CSTB/CST6/STFB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
104 |
99-108 |
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| EDTA plasma (n=4) |
98 |
93-105 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 20 |
2.411 |
2.588 |
2.500 |
2.340 |
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| 10 |
1.702 |
1.811 |
1.757 |
1.597 |
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| 5 |
1.166 |
1.163 |
1.165 |
1.005 |
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| 2.5 |
0.654 |
0.699 |
0.677 |
0.517 |
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| 1.25 |
0.423 |
0.411 |
0.417 |
0.257 |
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| 0.625 |
0.266 |
0.255 |
0.261 |
0.101 |
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| 0.313 |
0.203 |
0.211 |
0.207 |
0.047 |
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| 0 |
0.155 |
0.164 |
0.160 |
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货期
3-5 working days