产品描述
This Mouse BMP7 ELISA Kit was designed for the quantitative measurement of Mouse BMP7 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 pg/mL-40 pg/mL and the sensitivity is 0.156 pg/mL .
别名
Bmp7 ELISA Kit; Bmp-7 ELISA Kit; Op1 ELISA Kit; Bone morphogenetic protein 7 ELISA Kit; BMP-7 ELISA Kit; Osteogenic protein 1 ELISA Kit; OP-1 ELISA Kit
缩写
BMP7
Uniprot No
P23359
灵敏度
0.156 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse BMP-7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 90 | |
| Range % | 87-92 | |
| 1:2 | Average % | 103 | |
| Range % | 98-106 | |
| 1:4 | Average % | 92 | |
| Range % | 89-97 | |
| 1:8 | Average % | 100 | |
| Range % | 97-103 | |
回收率
| The recovery of mouse BMP-7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 94 | 91-96 | |
| EDTA plasma (n=4) | 95 | 93-98 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 40 | 2.449 | 2.440 | 2.445 | 2.295 | | | 20 | 1.788 | 1.818 | 1.803 | 1.653 | | | 10 | 1.196 | 1.181 | 1.189 | 1.039 | | | 5 | 0.584 | 0.604 | 0.594 | 0.444 | | | 2.5 | 0.424 | 0.415 | 0.420 | 0.270 | | | 1.25 | 0.321 | 0.312 | 0.317 | 0.167 | | | 0.625 | 0.272 | 0.255 | 0.264 | 0.114 | | | 0 | 0.152 | 0.148 | 0.150 | | |
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货期
3-5 working days