产品描述

This Human CD8A ELISA Kit was designed for the quantitative measurement of Human CD8A protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 U/mL-4000 U/mL and the sensitivity is 15.6 U/mL.

别名

alpha polypeptide (p32) ELISA Kit; CD8 ELISA Kit; CD8 antigen alpha polypeptide ELISA Kit; CD8 antigen alpha polypeptide (p32) ELISA Kit; CD8a ELISA Kit; CD8A antigen ELISA Kit; CD8A molecule ELISA Kit; CD8A_HUMAN ELISA Kit; Leu2 ELISA Kit; Leu2 T lymphocyte antigen ELISA Kit; Ly3 ELISA Kit; LYT3 ELISA Kit; MAL ELISA Kit; OKT8 T cell antigen ELISA Kit; OTTHUMP00000160760 ELISA Kit; OTTHUMP00000160764 ELISA Kit; OTTHUMP00000203528 ELISA Kit; OTTHUMP00000203721 ELISA Kit; p32 ELISA Kit; T cell antigen Leu2 ELISA Kit; T cell co receptor ELISA Kit; T-cell surface glycoprotein CD8 alpha chain ELISA Kit; T-lymphocyte differentiation antigen T8/Leu-2 ELISA Kit; T8 T cell antigen ELISA Kit; T8/Leu-2 T-lymphocyte differentiation antigen ELISA Kit

缩写

CD8A

Uniprot No

P01732

灵敏度

15.6 U/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Immunology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human CD8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		94
		Range %		86-98
1:2		Average %		99
		Range %		88-104
1:4		Average %		97
		Range %		89-100
1:8		Average %		105
		Range %		94-109

回收率

The recovery of human CD8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		100		88-104
EDTA plasma (n=4)		97		88-100

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
U/ml OD1 OD2 Average Corrected     4000 1.979 1.941 1.960 1.783     2000 1.471 1.455 1.463 1.286     1000 0.961 0.989 0.975 0.798     500 0.647 0.685 0.666 0.489     250 0.447 0.454 0.451 0.274     125 0.291 0.308 0.300 0.123     62.5 0.227 0.232 0.230 0.053     0 0.179 0.175 0.177

货期

3-5 working days