产品描述
This Rat Col Ⅱ Ab ELISA Kit was designed for the quantitative measurement of Rat Col Ⅱ Ab protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 0.312 U/mL-20 U/mL and the sensitivity is 0.078 U/mL.
别名
collagen type 2(Col 2) antibody,_x000D_
collagen type II(Col II) antibody
缩写
Col Ⅱ Ab
灵敏度
0.078 U/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat Col Ⅱ antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
89 |
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| Range % |
86-99 |
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| 1:2 |
Average % |
95 |
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| Range % |
91-100 |
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| 1:4 |
Average % |
93 |
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| Range % |
85-100 |
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| 1:8 |
Average % |
93 |
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| Range % |
82-98 |
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回收率
| The recovery of rat Col Ⅱ antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
96 |
91-110 |
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| EDTA plasma (n=4) |
98 |
89-100 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| U/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 20 |
2.607 |
2.725 |
2.666 |
2.498 |
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| 10 |
2.036 |
2.081 |
2.059 |
1.891 |
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| 5 |
1.328 |
1.369 |
1.349 |
1.181 |
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| 2.5 |
0.811 |
0.825 |
0.818 |
0.650 |
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| 1.25 |
0.436 |
0.421 |
0.429 |
0.261 |
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| 0.625 |
0.349 |
0.360 |
0.355 |
0.187 |
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| 0.312 |
0.243 |
0.232 |
0.238 |
0.070 |
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| 0 |
0.169 |
0.167 |
0.168 |
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货期
3-5 working days