产品描述
This Mouse 5-NT ELISA Kit was designed for the quantitative measurement of Mouse 5-NT protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.125 mU/mL-8 mU/mL and the sensitivity is 0.031 mU/mL.
别名
Nt5e ELISA kit; Nt5 ELISA kit; Nte5'-nucleotidase ELISA kit; 5'-NT ELISA kit; EC 3.1.3.5 ELISA kit; Ecto-5'-nucleotidase ELISA kit; CD antigen CD73 ELISA kit
缩写
5-NT
Uniprot No
Q61503
灵敏度
0.031 mU/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse 5-NT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 83 | | |
| Range % | 80-85 | | |
| 1:2 | Average % | 107 | | |
| Range % | 103-110 | | |
| 1:4 | Average % | 89 | | |
| Range % | 84-93 | | |
| 1:8 | Average % | 108 | | |
| Range % | 104-112 | | |
回收率
| The recovery of mouse 5-NT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 85 | 80-90 | | |
| EDTA plasma (n=4) | 100 | 95-105 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
|
| mU/ml | OD1 | OD2 | Average | Corrected | | | | 8 | 2.057 | 1.968 | 2.013 | 1.860 | | | | 4 | 1.397 | 1.402 | 1.400 | 1.247 | | | | 2 | 0.921 | 0.900 | 0.911 | 0.758 | | | | 1 | 0.575 | 0.601 | 0.588 | 0.435 | | | | 0.5 | 0.367 | 0.370 | 0.369 | 0.216 | | | | 0.25 | 0.255 | 0.261 | 0.258 | 0.105 | | | | 0.125 | 0.197 | 0.190 | 0.194 | 0.041 | | | | 0 | 0.150 | 0.156 | 0.153 | | | |
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货期
3-5 working days