产品描述
This Human 3-NT ELISA Kit was designed for the quantitative measurement of Human 3-NT protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL .
缩写
3-NT
灵敏度
0.039 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human 3-NT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 97 | |
| Range % | 92-102 | |
| 1:2 | Average % | 90 | |
| Range % | 82-98 | |
| 1:4 | Average % | 104 | |
| Range % | 99-111 | |
| 1:8 | Average % | 86 | |
| Range % | 82-96 | |
回收率
| The recovery of human 3-NT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 90 | 85-97 | |
| EDTA plasma (n=4) | 91 | 85-96 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml | OD1 | OD2 | Average | Corrected | | | 10 | 2.655 | 2.752 | 2.704 | 2.553 | | | 5 | 2.111 | 2.275 | 2.193 | 2.042 | | | 2.5 | 1.486 | 1.465 | 1.476 | 1.325 | | | 1.25 | 0.769 | 0.799 | 0.784 | 0.633 | | | 0.625 | 0.485 | 0.462 | 0.474 | 0.323 | | | 0.312 | 0.372 | 0.399 | 0.386 | 0.235 | | | 0.156 | 0.251 | 0.264 | 0.258 | 0.107 | | | 0 | 0.152 | 0.150 | 0.151 | | |
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货期
3-5 working days