产品描述
This Human 8-epi-PGF2α ELISA Kit was designed for the quantitative measurement of Human 8-epi-PGF2α protein in serum, urine, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 78 pg/mL-5000 pg/mL and the sensitivity is 19.5 pg/mL.
别名
8-epi-prostaglandin F2α,8-epi-PGF2α,8-epi-PGF2 alpha
缩写
8-epi-PGF2α
灵敏度
19.5 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human 8-epi-PGF2α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 96 | |
| Range % | 87-103 | |
| 1:2 | Average % | 86 | |
| Range % | 82-93 | |
| 1:4 | Average % | 100 | |
| Range % | 95-103 | |
| 1:8 | Average % | 93 | |
| Range % | 84-100 | |
回收率
| The recovery of human 8-epi-PGF2α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 96 | 90-103 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 5000 | 2.965 | 2.897 | 2.931 | 2.820 | | | 2500 | 2.211 | 2.323 | 2.267 | 2.156 | | | 1250 | 1.599 | 1.566 | 1.583 | 1.472 | | | 625 | 0.811 | 0.802 | 0.807 | 0.696 | | | 312 | 0.505 | 0.474 | 0.490 | 0.379 | | | 156 | 0.311 | 0.301 | 0.306 | 0.195 | | | 78 | 0.251 | 0.234 | 0.243 | 0.132 | | | 0 | 0.113 | 0.109 | 0.111 | | |
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货期
3-5 working days